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Top rated assay kits

AI-generated summary · updated July 27, 2026 · verify details before purchase.

  1. Best overall

    Thermo Scientific Pierce BCA Protein Assay Kit

    4.7/5 $300-$500

    Highly reliable and widely used protein quantification assay with excellent accuracy and reproducibility. Compatible with most detergents and reducing agents.

    • High accuracy and reproducibility
    • Works with many buffer types
    • Easy protocol
    • Requires incubation at 37°C
    • Not compatible with all chelating agents
  2. Best value

    Bio-Rad DC Protein Assay Kit II

    4.5/5 $150-$250

    Low-cost, detergent-compatible protein assay with a simple protocol and fast results. Ideal for routine lab work.

    • Cost-effective
    • Fast protocol
    • Good linearity
    • Color reagent can stain
    • Slightly less sensitive than BCA
  3. Best for cell viability

    Promega CellTiter 96 AQueous One Solution Cell Proliferation Assay (MTS)

    4.6/5 $200-$400

    Rapid, single-step cell viability assay that is more convenient than MTT. Requires fewer steps and less handling.

    • Single-step protocol
    • No washing steps
    • More sensitive than MTT
    • Works only with living cells
    • Reagent is light-sensitive
  4. Best for ATP detection

    Abcam ATP Assay Kit (Luminescence)

    4.4/5 $300-$500

    Highly sensitive bioluminescent assay for measuring ATP levels in cells or lysates. Used for cell viability, proliferation, and cytotoxicity studies.

    • Extremely sensitive
    • High signal stability
    • Plate-reader compatible
    • Requires luminometer
    • Expensive per test
  5. Best for western blotting

    Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate

    4.8/5 $100-$200

    Sensitive and stable chemiluminescent HRP substrate for western blot detection. Long-lasting signal and low background.

    • High sensitivity
    • Low background
    • Long signal duration
    • Only for HRP detection
    • Not for colorimetric use
  6. Best for glucose measurement

    Sigma-Aldrich MAK064 Glucose Assay Kit

    4.3/5 $100-$200

    Simple colorimetric assay for glucose quantification in biological samples. Fast and robust with a wide detection range.

    • Simple protocol
    • Wide detection range
    • Good specificity
    • Requires 37°C incubation
    • Color stable for limited time
  7. Best for cytokine detection

    R&D Systems Human TNF-alpha Quantikine ELISA Kit

    4.6/5 $400-$600

    Highly specific and sensitive ELISA kit for human TNF-α measurement. Validated for serum, plasma, and cell culture supernatants.

    • High specificity and sensitivity
    • Validated for multiple matrices
    • Easy protocol
    • Expensive
    • Single-plex only
  8. Best for creatinine measurement

    Cayman Chemical Creatinine Assay Kit

    4.2/5 $250-$350

    Colorimetric assay for creatinine in urine or plasma. Simple and fast with minimal sample preparation.

    • Fast results
    • Minimal sample prep
    • Good for high-throughput
    • Interference from bilirubin
    • Requires fresh samples
  9. Best for apoptosis detection

    Promega Caspase-Glo 3/7 Assay Kit

    4.5/5 $300-$500

    Bioluminescent assay for measuring caspase-3 and -7 activity in apoptotic cells. Highly sensitive and convenient for screening.

    • Sensitive and rapid
    • Homogeneous assay
    • Works with adherent and suspension cells
    • Expensive
    • Requires luminometer
  10. Best for pyruvate quantification

    BioVision Fluorometric Pyruvate Assay Kit

    4.1/5 $200-$300

    Sensitive fluorometric assay for pyruvate in biological samples. Suitable for metabolic studies.

    • High sensitivity
    • Simple protocol
    • No deproteinization needed
    • Requires fluorescence reader
    • Limited linear range

Buying advice

When choosing an assay kit, consider compatibility with your sample type and detection method (colorimetric, fluorometric, luminescent). Check sensitivity limits and dynamic range. Look for verified user reviews and batch-to-batch consistency. Prefer kits with clear protocols and active technical support. Balance cost per test against throughput needs.

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